2024/5/8/ · Packed bed perfusion bioreactors are widely applied in bottom-up tissue engineering due to their ability to control perfusion to tissues. Modeling and simulation software packages are used as powerful tools to predict the perfusion and flow distribution to the tissues and to design robust bioreactors.
2016/2/19/ · Nitrification performance (mg N L −1 d −1) and nitrogen loss (%), that is, the amount of removed NH 4 +-N not recovered as NO 2 −-N or NO 3 −-N, in the thermophilic bioreactor (50 °C) inoculated with thermophilic nitrifying batch enrichments from compost samples.Average influent NH 4 +-N and NO 2 −-N concentrations (mg N L −1, n=±70,
An important operational variable for continuous flow bioreactors is the dilution rate D, or the volume flow rate of the feed medium divided by the constant volume of the broth in the reactor.The biomass productivity of a continuous culture is simply the dilution rate D multiplied by the biomass concentration X in the harvest stream. In a continuous flow well
2015/1/1/ · Realistic numerical simulations demonstrate that the proposed observer based robust control strategy is able to maximize the biomass concentration despite large disturbances on the measurements (30 % for substrate and 15 % for biomass concentrations). Keywords: overflow metabolism, fed-batch bioreactor, robust control,
2014/9/11/ · Both projects showed consistent and robust cell culture performance in pilot-scale bioreactors (1,000 L) compared with the 5-L, 10-L, and 200-L bioreactors. Clinical manufacturing campaigns involving projects 2 and 5 demonstrated that the new platform process is scalable with minimal process deviations.
ORIGINAL ARTICLE A robust nitrifying community in a bioreactor at 50°C opens up the path for thermophilic nitrogen removal Emilie NP Courtens1, Eva Spieck2, Ramiro Vilchez-Vargas1, Samuel Bodé 3
2022/3/15/ · In this study, a continuous-flow bioreactor packed with well-organized lipase microarrays was developed for the sustainable synthesis of functional lipid
Robust I-PD Controller Design with Case Studies on Boiler Steam Drum and Bioreactor Abstract: Integral-proportional-derivative (I-PD) is more effective than PID controller as it provides dual-loop advantage while retaining the same number of tunable parameters.
2022/12/8/ · Bioreactors were inoculated with 100 ml seed culture and the continuous phase was initiated after a rapid increase in the pO 2 signal marked the end of the batch phase. During continuous operation mode, the medium was fed at a fixed rate of 2.83 ml min −1 to yield a dilution rate of 0.1 h −1 via mass balancing of the whole fermenter through
2016/3/25/ · The goal of this work is to present a class of state observer in order to infer unavailable concentrations in a sulfate-reducing bioreactor containing an anoxic culture of Desulfovibrio alaskensis 6SR from the measurements of the sulfate concentration. The methodology is applied to the corresponding sulfate-reducing model considering
2019/6/1/ · Systems and synthetic biology can be applied to design microbial strains that allow reliable and robust production on a large scale. Commercial microbial
2023/5/1/ · The robust coating and porous microstructure of QQ-ECHB contributed to keeping a lasting QQ activity and stable physical washing effect at a very low dosage (10g beads/5L MBR). Physical stability and environmental-tolerance tests also verified that the carrier can maintain the structural strength and keep the core bacteria stable when
2021/7/1/ · Querying this pooled P. putida mutant library could identify genes beyond metabolism that are required for robust growth in bioreactors. We extend this fitness profiling data to identify gene mutants implicated in improved bioreactor fitness, and then construct a small library of barcoded deletion strains. We then tested if deletion strains
2021/1/21/ · In this study, we selected the BioLector micro-bioreactor (µ-bioreactor) system as an HTP cultivation platform to screen E. coli expression clones producing …